منابع مشابه
Actin from Heart Muscle: Isolation, Purification, and Physicochemical Properties.
• A comparison of the properties of cardiac actin with those of skeletal actin has been undertaken in an attempt to explain several fundamental differences between the behavior of heart and skeletal muscle which have recently been elucidated. Most striking among these is the finding that cardiac muscle develops less tension than its skeletal counterpart. Burton has calculated that the force gen...
متن کاملAntigen Isolation and Purification
An antibody was raised against a protein isolated from the cytoplasm of mesothelioma cells. It was subsequently used in an immunoperoxidase procedure on formalin fixed, paraffin embedded tissue sections. A representative sample of benign and malignant tumours from all the systems of the human body was examined. All the tumours derived from coelomic surfaces (mesotheliomas of pleura, peritoneum,...
متن کاملIsolation and Purification of Dna
IMPORTANT NOTE: The smallest amount of contamination of DNA preparations by recombinant phages or plasmids can be disastrous. Many person-years have been wasted reisolating previously cloned sequences that contaminated preparations of DNA used to create recombinant DNA libraries and many researchers have been embarrassed to find that the “extra” genes they found on their Southern blots were act...
متن کاملReversible polymerization and ultracentrifugal purification of actin.
Actin is the second major structure protein of muscle. It is characterized by its ability to combine with myosin and to occur in two forms, globular and fibrous actin (9, 10, 3), which are indicated as Gand Factin. The transition of Ginto F-actin is brought about by addition of salt at neutral or weakly alkaline reaction (9, 10, 4). The present paper describes a method of purifying actin by pre...
متن کاملThe purification of choline acetyltransferase of squid-head ganglia.
Choline acetyltransferase (EC 2.3.1.6) isolated from the head ganglia of squid could be purified by use of mercurial-Sepharose columns as well as Sepharose columns to which the enzyme inhibitor p-(m-bromophenyl)vinyl pyridinium had been attached. These columns, in conjunction with 30-55% ammonium sulfate precipitation, 40-30% ammonium sulfate extraction, chromatography on sulfopropyl-Sephadex a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: NIPPON SUISAN GAKKAISHI
سال: 1977
ISSN: 1349-998X,0021-5392
DOI: 10.2331/suisan.43.877